Expression data from PBDE-47 treated rats at PND4

To identify liver transcripts differentially expressed between control samples and animals exposed to PBDE-47, we collected RNA from male pups at postnatal day 4 (PND4) after the Wistar Han dams were exposed to 0, 0.1, 15, or 50 mg/kg PBDE-47. These samples were interrogated with the Affymetrix Rat Genome 230 2.0 GeneChip array. A total of 7, 50, and 149 gene transcripts were differentially expressed between control samples and 0.1, 15, and 50 mg/kg PBDE-47 (using a false discovery rate (FDR) threshold of 0.05). These transcripts were mapped to 2, 40, and 139 genes using Ingenuity Pathway Analysis. There were 4 groups and 5x replication for each group, for 20 total samples. The groups were (1) vehicle control, (2) 0.1 mg/kg PBDE-47, (3) 15 mg/kg PBDE-47, and (4) 50 mg/kg PBDE-47. We compared vehicle control vs the dose group using the R/maanova package. The differentially expressed transcripts were identified based on a false discovery rate threshold of 0.05.