NLRP2 promotes proinflammatory and chemotactic responses in proximal tubular epithelial cells

In this study, we investigated the functional role of NLRP2 in proximal tubular epithelial cells by overexpressing it in human conditionally immortalized cells obtained from urine of a healthy subject. We found that NLRP2 markedly upregulates proinflammatory and chemotactic responses as well as downregulates interferon-mediated signaling pathways. mRNA profiles of conditionally immortalized proximal tubular epithelial cells (ciPTEC) stably overexpressing NLRP2 protein (NLRP2_over) and ciPTEC transfected with an empty vector (control, CTRL) were generated by deep sequencing, in biological duplicate, using Illumina HiSeq2000