Frequency of two PSbMV variants in pea leaves and seedlings in three sets of plants corresponding to three sampling designs.

For plants 1 to 19, leaves were sampled at 22 and 61 dpi; for plants 20 to 39, leaves were sampled only at 61 days post inoculation (dpi) and for plants 40 to 49, no leaves were sampled. n: total number of leaves or seedlings analyzed. Seedlings were analyzed only for plants that produced nine seeds or more. The percentages of the DPD1-R variant in inocula 1 and 2 were 37.8% and 65.9%, respectively. aMean relative frequency (×100) and standard deviation (×100; between parentheses) of the DPD1-R specific marker in the viral population. bFrequency of healthy seedlings (i), of seedlings infected by DPD1 (ii) or DPD1-R (iii) only, and of seedlings co-infected by both PSbMV variants (iv).