Antimicrobial susceptibilities for Mycobacterium tuberculosis Phsp60 TK strain are similar to the wild-type parent strain.

M. tuberculosis H37Rv and M. tuberculosis Phsp60 TK strains were tested against common anti-TB drugs (streptomycin, ethambutol, INH, rifampin and moxifloxacin) using the standard broth dilution method. Mid-log phase bacteria were diluted to a concentration of 100,000 organisms/ml and grown at 37°C in Middlebrook 7H9 liquid broth (without Tween 80) containing different concentrations of antibiotics. Mean inhibitory concentrations (MIC) were determined 10–14 days later. As shown, there is no significant difference between MICs for 5 common anti-TB drugs for either strain.