Transcriptomic Responses of Mycoplasma bovis upon Treatments of trans-Cinnamaldehyde, Carvacrol, and Eugenol

Through RNA sequencing, we report here that Carvacrol (CAR) affected the expression of 153 genes which included the down-regulation of energy generation-related proteins, pentose phosphate pathway, and up-regulation of ribosomes and translation-related proteins. Few differentially expressed genes were found when M. bovis were treated with Trans-cinnamaldehyde (TC), Eugenol (EU), or Carvacrol (CAR) when the three PDAs were double or triple combined. Our results suggest that, as opposed to the effect of CAR, the growth inhibitory effects of TC and EU at levels tested may be exerted through mechanisms other than gene expression regulations. Overall design: Examining the effect of Trans-cinnamaldehyde, Carvacrol, and Eugenol on Mycoplasma bovis