Localization and degradation data of the Arabidopsis borate transporter BOR1 in tobacco BY-2 cells

Borate effect on BY-2 growth.csv
This is the dataset for Figure 2 in the associated article. Seven-day old tobacco BY-2 cells were subcultured into medium containing the indicated concentration of borate (mM) and shaken for a week. The volume of cells in the culture was measured after sedimentation of the cells by centrifugation. Packed cell volume indicate the actual volume of sedimented cells (ml) and the relative value represent the relative volume of the average of the triplicated experiment at 0.1 mM borate condition, which corresponds to the normal growth condition. Average of relative value and STDEV were used to make Figure 2.
BOR1-GFP localization in BY-2 cells.csv
This is the dataset for Figures 4C and 8B in the associated research article. The column A “cell type” indicates the cell line and collected image name. PG represents the PIP-GFP, WT represent the wild-type BOR-GFP, and mut represent the mutant BOR-GFP. The numbers after PG, WT and mut indicate the image number. Column B to I represent the actual data collected for the analysis. At each face of the cell images a box was made on the region corresponds to either of the face of the cells and the density of the intensity in the box was quantified (e.g. column B, A1). The same size box was copied close to the box at the outside of the cell and used to quantify the background intensity and the density of the intensity in the box was quantified (e. g. column C, A1 BG). Column J to M represent the subtracted density of fluorescence from the face to background (e. g. column B value minus column C value). The coumn N to Q represent the relative value of intensity to E1 face. The A1, A2, E1, E2 faces are indicated in Figure 4B.
Borate medium conc BOR1-GFP degradation.csv
This is the dataset for Figure 3C in the associated research article. Time course analysis of the degradation of BOR-GFP was quantified from the gel image. The column A indicate the experiment No. and the relative value of each experiments to time 0 as well as the average and standard deviation (STDEV) value of the relative intensity to time 0. From column B to K the top row indicate the time point and the other row indicate the actual and relative values, average and STDEV of relative to 0 time intensity.
CHX BOR1-GFP degradation.csv
This is the dataset for Figure 5B. Images were quantified from the gel and the relative value to without borate was calculated as described in the legend to . Column A is the experiment No.
Colum B to I are the relative values of each treatment described in row A of the column. The numbers in the top row of columns B to I are the concentration of cyclohexamide (mM).
Time course inhibitors BOR1-GFP degradation.csv
This is the dataset for Figure 7B in the associated research article. In each experiment, to analyze the effect of TIBA or tyrphostin A23, a control without both borate and inhibitor was used. The relative value of the intensity of the bands were quantified as in the legend to . The values of row 1-4 are identical to the relative value of .
Borate dependent degradation inhibition.csv
This is the dataset for Figure 7A in the associated article. Column A indicates the experiment No. and the average and STDEV of the relative value. Column B indicates whether the data is the actual intensity or is the relative intensity to the values without both borate and inhibitors. The top row of column C to O indicates the presence and absence of borate and the names of inhibitors used. From the second to 25th row of columns C to O, the actual value as well as the relative values for without both borate and inhibitors were shown.
Relative amount of Bor1-GFP at the addition of inhibitor (from time-course data).csv
This is the background dataset for the processing of the data for Figure 7D in the associated research article. At the zero time or indicated time inhibitors were add to the culture. The relative intensity of bands to the start of the incubation with borate (0 time) is shown. Based on the average value in this dataset the relative value of the intensities of the bands in were calculated.
Timimg of the addition of inhibitors (Fig 7D).csv
This is the dataset for Figure 7D in the associated article. The relative values against each time points are shown. The time written in the first row indicates the timing of the addition of inhibitors. After calculation of the relative intensity to the time 0 without borate value as calculated in , the average and STDEV of three experiments were calculated. Thereafter these values were normalized to the average value calculated in (rows 7 and 8) and used to make Figure 7D.

{'Borate_effect_on_BY-2_growth.csv': '本数据集为相关文章中图2的数据集。将七日龄的烟草BY-2细胞接种于含有指定浓度硼酸（mM）的培养基中，摇匀培养一周。通过离心使细胞沉淀，测量培养液中细胞的体积。填充细胞体积（ml）表示实际沉淀细胞的体积，相对值代表在0.1 mM硼酸条件下的三份重复实验的平均相对体积，该条件对应于正常生长条件。通过平均相对值和标准差（STDEV）绘制图2。', 'BOR1-GFP_localization_in_BY-2_cells.csv': '此数据集为相关研究文章中图4C和8B的数据集。列A中的“细胞类型”表示细胞系和收集的图像名称。PG代表PIP-GFP，WT代表野生型BOR-GFP，mut代表突变型BOR-GFP。PG、WT和mut之后的数字表示图像编号。列B至I代表实际收集用于分析的数据。在细胞图像的每个面上，绘制一个与细胞面相对应的区域框，并量化框内强度的密度（例如，列B，A1）。在细胞外靠近框的同一大小框被复制并用于量化背景强度，框内强度的密度也被量化（例如，列C，A1 BG）。列J至M代表从细胞面到背景的荧光强度减去后的密度（例如，列B值减去列C值）。列N至Q代表强度相对于E1面的相对值。A1、A2、E1、E2面如图4B所示。', 'Borate_medium_conc_BOR1-GFP_degradation.csv': '此数据集为相关研究文章中图3C的数据集。通过凝胶图像对BOR-GFP降解的时效性进行了量化分析。列A表示实验编号以及每个实验相对于时间0的相对值，以及相对于时间0的相对强度的平均值和标准差（STDEV）。从列B至K，最上行表示时间点，其他行表示实际值和相对于0时间点的相对值、平均值和标准差。', 'CHX_BOR1-GFP_degradation.csv': '此数据集为图5B的数据集。从凝胶中量化图像，并按照图例中描述的方法计算相对于无硼酸的相对值。列A是实验编号。列B至I是列A中A行描述的每种处理的相对值。列B至I的上行数字表示环己酰胺（mM）的浓度。', 'Time_course_inhibitors_BOR1-GFP_degradation.csv': '此数据集为相关研究文章中图7B的数据集。在每个实验中，为了分析TIBA或tyrphostin A23的效果，使用了一个不含有硼酸和抑制剂的对照实验。按照图例中描述的方法量化了带强度的相对值。行1至4的值与的相对值相同。', 'Borate_dependent_degradation_inhibition.csv': '此数据集为文章中图7A的数据集。列A表示实验编号以及相对值的平均值和标准差（STDEV）。列B表示数据是实际强度还是相对于无硼酸和抑制剂的相对强度。列C至O的上行表示硼酸和抑制剂的共存与否以及所使用的抑制剂名称。从第二行至第25行，列C至O显示了无硼酸和抑制剂的实际值以及相对值。', 'Relative_amount_of_Bor1-GFP_at_the_addition_of_inhibitor_(from_time-course_data).csv': '此为处理相关研究文章中图7D数据集的背景数据集。在零时间或指定时间向培养液中添加抑制剂。显示相对于硼酸培养开始（0时间）的带相对强度。基于此数据集中的平均值，计算了图中所示的带强度的相对值。', 'Timing_of_the_addition_of_inhibitors_(Fig_7D).csv': '此数据集为相关研究文章中图7D的数据集。显示了相对于每个时间点的相对值。第一行中写的时间表示添加抑制剂的时机。在计算相对于无硼酸的0时间点的相对强度后，计算了三个实验的平均值和标准差。然后，将这些值归一化到计算出的平均值（行7和8），并用于绘制图7D。'}