Elucidating transcriptional regulation of nitrogen metabolism with systems approaches [RNA-Seq]

NtrC and Nac have been known to be responsible for responding to nitrogen limitting conditions. In order to elucidate NtrC and Nac regulons in a genome-wide manner, RNA-seq and ChIP-exo experiments were performed for those two transcription factors under 4 different nitrogen sources, ammonia, glutamine, cytidine and cytosine. A total of eight samples were analyzed. E. coli K-12 MG1655 WT strain was cultured in the 4 different nitrogen sources, ammonia, glutamine, cytidine and cytosine, and glucose carbon source with biological duplicates.