Epigenetic profiling of sorted tumor cells from implanted T cell low and T cell high mouse pancreatic tumors

Purpose: Use ATAC-seq strategy to characterize the differentially openned chromatin region in T cell low and high mouse pancreatic tumors in order to identify factors contributing to the differential abundance of tumor infiltrating T cells. Methods: Subcutaneously implanted T cell low and high pancreatic tumors were utilized for the preparation of ATAC-seq libraries from sorted tumor cells. Examination of differentially openned chromatin region between two biological groups (T cell low v.s. T cell high tumors).