Impact of seeding density + fibroblast passage number on cell toxicity.

NIAID Data Ecosystem2026-03-08 收录

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https://figshare.com/articles/dataset/_Impact_of_seeding_density_fibroblast_passage_number_on_cell_toxicity_/900313 数据链接链接失效反馈

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Impact of seeding density + fibroblast passage number on cell toxicity.

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2014-01-13

Flux of major metabolites consumed and produced by human embryonic stem cells cultured at physiological and atmospheric oxygen concentrations.

细胞代谢研究

细胞培养条件优化

Note: All data measured by HPLC analysis, except for ammonia, which was measured using the Bioflex analyser. Values are mean ± standard error, n = 6. Significance between the values measured at 20% an

NIAID Data Ecosystem60

Identification of oxygen-sensitive transcriptional programs in human embryonic stem cells.

细胞培养条件优化

转录组学分析

To realize the full potential of human embryonic stem cells (hESC), it is important to develop culture conditions that maintain hESC in a pluripotent, undifferentiated state. A low O2 atmosphere (~4%

NIAID Data Ecosystem40

Characterization of 253G1 cells subcultured on laminin-521 in Essential 8 medium.

细胞多能性评估

细胞培养条件优化

(A) Expression levels of undifferentiated cell markers (OCT3/4, NANOG, SOX2 and LIN28) in 253G1 cells subcultured on laminin-521 in Essential 8 were determined using qRT-PCR. Relative mRNA expression

Figshare2016-02-23 更新20

Global transcriptional analysis of xeno-free and feeder-cultured human extended pluripotent stem cells by single cell RNA-seq. Global transcriptional analysis of xeno-free and feeder-cultured human extended pluripotent stem cells by single cell RNA-seq

细胞培养条件优化

单细胞转录组学

Extended pluripotent stem (EPS) cells have shown great applicative potentials in generating synthetic embryos, directed differentiation and disease modeling. However, the lack of a xenofree culture co

NIAID Data Ecosystem50

Comparison of gene expression in human embyronic stem cells grown in MEF-CM or defined media conditions

细胞培养条件优化

干细胞基因表达

A fully defined media for human embryonic stem cells (hESCs) was designed after examining the phosphorylation status of 42 receptor tyrosine kinases (RTKs) on hESCs exposed to mouse embryonic fibrobla

NIAID Data Ecosystem20

Impact of seeding density + fibroblast passage number on cell toxicity.

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