Transcriptional landscape of Camellia sinensis roots at single- nucleus resolution

In summary, this study identifies most cell types in the root tips of the tea plant through single-nucleus transcriptomic analysis, establishing marker genes and cell type-specific regulatory programs. The evolutionary conservation and divergence in cell type and species-specific gene expression between tea plant and Arabidopsis provide new insights into the functions and evolution of cell types in tea plant. And it provides a detailed transcriptomic overview of the main cell types at the root tips of the tea plant at single-nucleus resolution. Overall design: To conduct snRNA-seq analysis on tea plant roots, nuclei were extracted and purified from the tender root tips of the tea plant. Initially, the raw data were processed using Cell Ranger, the official analysis software of 10x Genomics, for data filtering, alignment, quantification, and identification of recovered cells, ultimately yielding a gene expression matrix for each cell.