Activity states of Primnoella chilensis colonies under sediment exposure of 15 days, Control, X-Huinay

To determine behavioural stress, polyp activity states of Primnoella chilensis colonies and the frequency of these states were observed under turbid seawater in an experimental setup for 15 days (Treatment 1: equals sedimentation rate of 225 mm/y; Treatment 2: equals sedimentation rate of 450 mm/y; control group: ambient seawater). A visual overview analysis of colonies of Primnoella chilensis was conducted with the image-viewing program IrfanView. In a defined sector the overall colony activity was assessed and categorized: category 0 = > 50 % of polyps closed; category 1 = > 50 % of polyps semi-open; 2 = > 50 % of polyps open ; x = no data.