Pharmacokinetic measurements of BRP levels following BRP administration in mice

Raw LC-MS data (raw.d files) generated on an Agilent 6470 Triple Quadrupole LC-MS instrument to provide evidence for the pharmacokinetic measurements of BRP peptide following administration in C57BL/6J male mice. Plasma and brains were collected 0, 1,10, 30, or 60 minutes after injection of BRP (10 mg/kg I.P.). By LC-QQQ, we successfully detected endogenous peaks with transitions corresponding to the parent ions (+2 and +3 charge) in mouse plasma and brain co-eluting at 14-15 minutes with the synthesized standard. Data also show the detection of two cleaved fragments from BRP, THRIL and LFNLC, in the plasma.

原始液相色谱-串联质谱（LC-MS）数据（raw.d文件），由Agilent 6470三重四极杆LC-MS仪器生成，旨在为C57BL/6J雄性小鼠注射BRP肽后的药代动力学测量提供证据。在注射BRP（10 mg/kg，腹腔注射）后0、1、10、30或60分钟收集血浆和脑组织。通过液相色谱-串联质谱（LC-QQQ），我们成功检测到与母离子（+2和+3电荷）对应的内源性峰，这些峰在14-15分钟与合成的标准品共流出。数据还显示，在血浆中检测到BRP的两个裂解片段，即THRIL和LFNLC。