Identification of differentially expressed proteins in response to SL treatment a.

aRice d10 mutant XJC seedlings were germinated and grown on medium in the presence of 1.0 μM GR24 for 6 days in darkness. Seedlings grown without exogenous application of GR24 served as a control. Total proteins were extracted from seedlings and resolved by 2-DE. Protein spots that exhibited differences in intensity were excised for identification by mass spectrometry.bRelative molecular mass (Mr) in Da and isoelectric point (pI) of proteins are theoretical.cNumber of matched peptides (MP).dSequence coverage (SC, %) of the identified peptides.eProteins with a Mowse score of more than 61 were considered to be identified at a statistically significant level. Significance threshold pfExpectation scores are the probability-based values obtained from database search by MASCOT. Values less than their threshold (pgProtein spot changes after GR24 treatment was indicated by up-regulation (Up), down-regulation (Down) and disappearance (DA).hFold changes (FC) with standard errors (±SE) from three biological replicates were calculated as the ratio of normalized spot intensities between the treatment and control.iFold changes (FC) in protein spot intensity with a p-value of less than 0.05 were considered to be statistically significant.