Ivo Zemp, Thomas Wild, Marie-Francoise O'Donohue, Franziska Wandrey, Ulrike Kutay, Pierre-Emmanuel Gleizes, Barbara Widmann (2011) CIL:13386, Homo sapiens. CIL. Dataset

This image is part of a group of 4 images supporting the conclusion that hRio2 kinase activity is required for cytoplasmic 18S-E pre-rRNA processing. This image is a control for the experiment and shows the distribution of all 18S rRNA precursors (white), via fluorescence in situ hybridization (FISH) with a 5' ITS1 probe, upon depletion of a protein important in rRNA processing, hRio2,in Hela cells. Depletion of hRio2 leads to the accumulation of 18S-E pre-rRNA in the cytoplasm (white). For the set of four images in this group, FISH pictures were processed in parallel by enhancing levels, followed by setting gamma correction to 0.75.

本图像系一组支持结论的证据，该结论认为hRio2激酶活性对于细胞质中18S-E前体rRNA的处理至关重要。该图像作为实验的控制组，展示了在Hela细胞中，通过荧光原位杂交（FISH）使用5' ITS1探针对hRio2（一种在rRNA处理中起重要作用的蛋白质）耗竭后的所有18S rRNA前体的分布（以白色呈现）。hRio2的耗竭导致细胞质中18S-E前体rRNA的累积（以白色呈现）。对于本组中的四幅图像，通过并行增强图像对比度，随后将伽马校正设置为0.75对FISH图像进行了处理。