Phase-contrast image dataset for morphological evaluation of SH-SY5Y differentiation after rosmarinic and retinoic acid treatments

This dataset contains raw phase-contrast microscopy images, image-level metadata, an ilastik pixel-classification project/model, ilastik training images, preprocessed ilastik training images, example expected ilastik outputs, and compact representative analysis outputs generated during morphological analysis of SH-SY5Y cells treated with rosmarinic acid and retinoic acid at different treatment concentrations, FBS concentrations, cell seeding densities and treatment times. The dataset supports the expected publication entitled "Morphological evaluation of the differentiation status of SH-SY5Y cells upon rosmarinic and retinoic acid treatments". The associated image-analysis workflow is designed to quantify neurite-associated morphology from phase-contrast images, including segmentation-derived neurite, cell-body, and background regions, ROI-level neurite features, skeleton-derived features, image-level summaries, and downstream statistical/visual interpretation.