Characterization of biological pathways regulating acute cold resistance of zebrafish - experiment 2

This experiment was performed to investigate the functional mechanisms of the critical pathways including ERK, p38 MAPK and the transcription factor Foxo1 in enhancing cold resistance of zebrafish.Specific inhibitors of these pathways were used to treat zebrafish larvae under both normal and low temperatures to investigate the functions of these pathways in regulating cold stress response. Zebrafish larvae were maintained at 28 oC or exposed to 10 oC for 12 h in the presence of inhibitors including AS1842856 (0.5 μM), PD0325901 (5 μM) and SB203580 (50 μM), respectively. An untreated control group (96 hpf) and a vehicle (0.1% DMSO) control were also included.