Gene expression profiling of Drosophila melanogaster larval brains after chronich alcohol exposure

We sequenced mRNA extracted from brains of (1) D. melanogaster larvae exposed to food containing 5% ethanol (v/v) for 6 conscutive days, and (2) an age-matched untreated control larvae, that grew in regular food. Differential gene expression between the two groups was calculated and reported. Each group consisted of 3 biological replicates of 30 brains each. Examination of mRNA levels in brains of D. melanogaster larvae after chronich ethanol exposure was performed using next generation sequencing (NGS) technology (RNA-seq).