Biofilm and sediment microbiota amplicon metagenome from an intermittent stream

Amplicon metagenome analysis to describe biofilm microbial communities from cobbles and sediment samples taken from an intermittent third order stream. Raw sequences from 16S rRNA amplicon sequencing (hiper-variable V4 region). The present data set consists of samples collected during the hydrological year 2019-2020, in spring (28 March), summer (6 June), fall (28 November), and winter (20 February) in Fuirosos (41.684583, 2.579444), an intermittent third order stream located within the Montnegre-Corredor Natural Park (Catalonia, NE Spain). For each season, samples were collected along a 100 m reach, divided longitudinally into 7 sections. In each section, two biofilm streambed sediment samples (i.e., epipsammon, 0-3 cm depth) were collected. Surface sediment samples (20-30 g each) were carefully transferred to 50 mL falcon tubes with 5 mL of lysis buffer (40 mM EDTA; 50 mM Tris pH 8.3; 0.75 M sucrose). In addition, we collected two cobbles (one located in a run zone and one in a riffle zone) to obtain epilithon samples at stations 1, 3, 5, and 7. In the laboratory, we extracted the epilithon from each cobble by scraping with a sterilized metal brush and washing them with ringer's solution. An aliquot of the sludge was filtered through a 0.2 micro-meters polycarbonate membrane and frozen at -20C degrees until subsequent microbial DNA extractions. The results were used to characterize the microbial community structure and to compare possible differences between seasons.