File S1 - Analysis of the Functional Interaction of Arabidopsis Starch Synthase and Branching Enzyme Isoforms Reveals that the Cooperative Action of SSI and BEs Results in Glucans with Polymodal Chain Length Distribution Similar to Amylopectin

Supporting information including tables S1–S2 and figures S1–S7 can be found in File S1. Table S1. Cloning of AtSSI-AtSSIV, AtBE2, and AtBE3. Table S2. rSSI activity in radiolabelled assays. Figure S1. SSI activity throughout the diurnal cycle. Figure S2. SS activities of be knock-out plants. Figure S3. Mobility of SSI, BE2, and BE3 protein in native PAGE using different knock-out lines. Figure S4. CLD profiles of glucans extracted from rSSI, rBE2, and rBE3 preparations. Figure S5. Comparison of rSSI/rBE3 interaction in presence and absence of citrate. Figure S6. Effect of BE amount on rSSI/rBEs interaction. Figure S7. Difference plots of CLDs from glucans generated by SSI/BE interaction in-gel and Arabidopsis starch. (PDF)