Gene expression alterations with Nkapl knockout or NKAPL frameshift mutation (M349fs). Gene expression alterations with Nkapl knockout or NKAPL frameshift mutation (M349fs)

This study aims to explore the transcriptomic changes in Nkapl-KO, Sox30-KO and Nkapl TGdel/TGdel mouse testes comparing with their wild-type testes. Fresh testes were collected from 21d wild-type, Nkapl-KO, Sox30-KO and Nkapl TGdel/TGdel mice, respectively. Overall design: For each group, we have biological triplicates. Total RNA was extracted using TRIzol reagent. Strand-specific libraries were prepared using the TruSeq Stranded Total RNA Sample Preparation kit (Illumina) according to the manufacturer's instructions.