Overview of the effect of mutations in the Apobec3G MAPPIT bait on the interaction with the Gagpol prey.

MAPPIT luciferase fold inductions for each mutant are expressed as percentage of wild type Apobec3G bait. The averages of several independent MAPPIT experiments and standard deviation (SD) are shown. Strongly decreased values that are significantly different from the WT in a paired t-test are indicated by an asterisk. HEK293T cells were transiently co-transfected with plasmids encoding the chimeric Apobec3G WT or mutant Apobec3G bait constructs and Gagpol prey constructs, combined with the pXP2d2-rPAP1-luci reporter. The transfected cells were either stimulated for 24 h with Epo or were left untreated. Luciferase measurements were performed in triplicate. Data are expressed as a percentage of the WT fold inductions (Epo-stimulated/unstimulated).