Effect of Ligands on the Cross-Linking of Glut1 Di-cysteine Mutants.

Oocyte membranes expressing dicysteine mutants were subjected to chemical cross-linking as described in Materials and Methods in the presence or absence of cytochalasin B (CB) or ethylidene glucose (EG). Cross-linking efficiency is expressed as the intensity of the full-length transporter bands in the cross-linked lanes (o-PDM and BMH lanes, see Figure 4) divided by the intensity of the full-length transporter bands in the control lanes, after subtraction of background intensities. See Figure 4 for a representative set of immunoblots. The results of 4–5 independent experiments are presented. Ethanol (ETOH) is the control for the addition of cytochalasin B (CB), and Water is the control for the addition of ethylidene glucose (EG).