qChIP analysis of H3K27ac at Creb5 and Skap2 which lack LncRNA-HIT associated peaks.

(A)Creb5-assocaited H3K27ac peaks assessed for changes in fragment enrichment in response to LncRNA-HIT siRNA treatments. (B) qChIP analysis of H3K27ac peak enrichment in response the LncRNA-HIT siRNA treatments at the Creb5 locus. Values represent the mean fold change H3K27ac fragment enrichment relative to parallel treatments using a scrambled siRNA control. Bars represent the standard deviation of the mean for three independent assays. (C) Skap2-associated H3K27ac peak assessed for changes in fragment enrichment in response to LncRNA-HIT siRNA treatments. (D) qChIP analysis of H3K27ac peak enrichment in response the LncRNA-HIT siRNA treatments at the Skap2 locus. Value represents the mean fold change H3K27ac fragment enrichment relative to parallel treatments using a scrambled siRNA control. Bar represents the standard deviation of the mean for three independent assays.