Community composition, symbiotic patterns and influencing factors of planktonic fungi in Nanchang Lake

Abstract: [Objective] To analyze the temporal changes of planktonic fungal community composition and symbiotic patterns in Nanchang Lake, and to reveal the factors influencing the temporal changes of planktonic fungal community composition and symbiotic patterns. [Methods] To analyze the distribution characteristics of environmental factors such as pH, dissolved oxygen (DO), total dissolved solids (TDS), water temperature (WT) and ammonia nitrogen (NH4+-N) in Nanchang Lake. High-throughput sequencing was used to characterize the planktonic fungal communities, PLS-DA analysis was used to determine temporal differences in planktonic fungal communities, Mentor test was used to investigate the relationship between environmental factors and planktonic fungal community composition, network analysis was used to determine the differences in symbiotic patterns between different water periods, and Module-EigenGene analyses were used to investigate the relationship between environmental factors and topological characteristics of symbiotic patterns. [Results] The structure of the planktonic fungal community in Nanchang Lake varied significantly between water periods, and the endemic species differed significantly between dry and abundant water periods. WT was the best environmental factor affecting the symbiotic pattern of planktonic fungal communities. [Conclusion] The composition of planktonic fungal communities differed significantly between water periods, and temperature was the best environmental factor to explain the changes of planktonic fungal community structure and symbiotic patterns between water periods. This study aims to provide a theoretical basis for the comprehensive evaluation and management of lakes, and to provide guidance for the protection of lake ecosystems in the middle and lower reaches of the Yangtze River.