Six in situ Hi-C libraries of control and mESC auxin-inducible degrons for Scc1 and Ring1b.
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https://www.ncbi.nlm.nih.gov/sra/ERP114475
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In this experiment, we've examined chromatin conformation differences of E14 mESC against cohesin and Ring1b degrons. We performed a modified in situ Hi-C protocol from (Rao et al., 2014) that can be found in detail at (DÃaz et al., 2018). Samples were digested with MboI restriction enzyme. The aim of the experiment was to characterize the role of cohesin and polycomb on the 3D structure of mouse chromatin.
创建时间:
2023-10-13



