Gene Expression Profile in the Hypothalamus of Male Bgn_KO and WT Mice at 13 Weeks Old. Mus musculus

To investigate the effects of gene Bgn on affecting brain dysfunction and metabolic disorders by profiling the transcripome in the hypothalamus of male Bgn_KO and wild-type (WT, litter mate) mice at 13 weeks old. Overall design: All mice (male Bgn_KO (n=4) and WT (n=4)) were maintained in a temperature-controlled room with a 12-hour light and 12-hour dark cycle. Diet (Pico Lab 5053 rodent diet) and water were provided ad libitum. At 13 weeks old, mice were sacrificed. Hypothalamus and liver were dissected out, flash frozen, and stored at -80°C for later usage. All experiments were performed in accordance with the United States National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the University of California at Los Angeles Chancellor?s Animal Research Committee.