High resolution mapping of common fragile sites in the human genome through sequencing of sites of mitotic DNA synthesis

Cells encountering DNA replication stress often fail to fully replicate their genome prior to mitotic entry, which leads to genomic instability at specific genomic loci, the so-called common fragile sites(CFSs). Mitotic DNA synthesis (MiDAS) is a DNA repair pathway used by the cells in early mitosis to complete the duplication of unreplicated genomic sites. Here, we used high-throughput sequencing in order to map sites of MiDAS at high resolution, genome-wide, in three different cell lines treated with aphidicolin.