MDCK cells were infected at MOI 0.05 with the indicated viruses. Supernatants were collected at 1, 8, 24 and 48 hours post infection and viral titers were quantified by TCID50. Images show the TCID50
Statistical analysis indicates the significant difference in carriage of virulence factors (based on a PCR survey) and lethal effects (Low LD50) with larvae inoculated with ST69 and ST127 strains.
Data for Figs 1 and 4 (Table 1): Titers (PFU/ml) of intermediate stocks grown in Vero cells inoculated with MOI=0.01 with either recovered transfection supernatants or wild type stock viruses. Titers
aAfter autopsy on indicated days after virus infection, lung tissue samples were collected.The lung tissue homogenates were prepared and used to infect MDCK cells to determine TCID50 per gram of tissu
