Sequencing using pulsed field and image reconstruction.
收藏PubMed Central1993-11-01 更新2026-05-16 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC47721/
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资源简介：
The use of pulsed fields in a standard manual sequencing set-up results in the separation of > 2 kb on a single gel, as compared to 300-400 bases with a dc field. However, visual reading of the sequence from a film exposed to a pulsed-field gel is not possible for more than 800-900 bases under the best conditions. The use of image reconstruction and enhancement techniques allows the reading of the M13mp18 sequence to > 1 kb, and individual bands can be identified at > 2 kb. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1993-11-01
