Genetic analysis and identification of the candidate genes of maize resistance to Ustilago maydis by BSA-Seq and RNA-Seq

Maize common smut is a common disease caused by Ustilago maydis, can cause serious damage to the yield and quality of maize. To elucidate the genetic mechanism of resistance to maize smut disease, and to discover the relevant resistance genes, which have important scientific implications for maize resistance breeding. This study used the high-resistance maize variety Qi319 and the high-susceptibility variety Ye478 as materials, and constructed different segregating populations through hybridization, backcrossing, and other methods. Through field artificial inoculation and disease resistance identification, the six population (P1, P2, F1, F2, BC1P1, BC1P2) constructed was subjected to genetic and correlation analysis using a plant "quantitative trait main gene + multi gene mixed genetic model".