Brucella NyxA and NyxB dimerization enhances effector function during infection

Supporting data (uncropped RAW western blots) for the article entitled: Brucella NyxA and NyxB dimerization enhances effector function during infection Data concern two Brucella translocated effectors, NyxA and NyxB, that contribute to the late stages of intracellular multiplication. We show that 1) NyxA and NyxB directly interact; 2) monomeric forms of the Nyx effectors still interact with their host cellular target, the deSUMOylase sentrin-specific protease 3 (SENP3); 3) monomers are less able to delocalize SENP3 from the nucleoli.