Gene expression divergence in experimentally evolved Drosophila pseudoobscura females

We used experimentally evolved Drosophila pseudoobscura females, who have experienced either enforced monogamy or increased polyandry for 100 generations, to assess divergence in their transcriptomic response to both mating system variation and mating experience. Gene expression was measured from 5-days old females from two experimentally evolved lines (M=monogamous, P=elevated polyandrous). We measured gene expression of females from each of the two selection lines 24h-h after mating with either M or with P males, or as virgins. The same design was repeated for two replicate selection lines per treatment, and within each we obtained three independent biological replicates per treatment category.