Zinc-induced pancreatic pathology in control and Zip5 Panc KO mice.

Newly weaned mice were injected with tamoxifen to induce Zip5 recombination in pancreatic acinar cells by activation of Cre:ERT2 expressed from an ela-Cre-ERT2 transgene. Two weeks after the last tamoxifen injection, control (Control) littermates and pancreas-specific Zip5-knockout (Zip5 Panc KO) were given an I.P. injection of zinc sulfate (6.25 mg zinc/kg body weight) and 24 h later pancreata were harvested and paraffin sections were prepared, stained with hematoxylin and eosin and evaluated blind by a pathologist. Each row represents an individual animal.