Lentiviral single-cell MPRA of synthetic enhancers in hematopoiesis

R data objects associated with Rühle et al., biorxiv 2026. Three enhancer libraries (PoC, alpha, beta) were screened by single cell MPRA in primary hematopoeitic stem and progenitor cells (HSPCs). Library PoC was also screened in K562 cells.Seurat objects (targeted gene expression data)integrated_seurat.rds : Integrated gene expression data for the HSPC screens of library alpha and beta. Metdata column experiment indicates which library was used. final_annotation contains final cell type/cell state annotation. SCT_snn_res.* columns contain different clustering resolutions. In the correlation-based analyses from the manuscript, resolution SCT_snn_res.2 was used.libPoC_hsc_seurat.rds : Gene expression data for the HSPC screen of library PoClibPoC_k562_seurat.rds : Gene expression data for the K562 screen of library PoCEnhancer activity dataData frames containing, for each observed combination of presence barcodes (guide_id) and cell (cell), information on the UMI count of the detection barcode (guide_count), the UMI count of the quantification barcode (gfp_count). Entries in the cell column correspond to column names of the Seurat objects. Additionally, contains information on the enhancer including the sequence, a sequence ID (CRS), and several column describing the sequence design, such as the identities of the transcription factors used (TF.1, TF.2), their affinity, number of repeats, spacing, etc., and a systematic text description of the design (Seqname)libAlpha_matched_gfp_guide_enhancer_counts.rdslibBeta_matched_gfp_guide_enhancer_counts.rdslibPoC_hsc_gfp_guide_enhancer_counts.rdslibPoC_k562_gfp_guide_enhancer_counts.rds