Transcriptomic profiling and analyses of murine prostate orthografts

RNA was extracted from the frozen prostate tumours or tissues of P1 prostate tumours (n=3), CP1 prostate tumours (n=3) and CP2 prostate tumours (n=4). RNA quality of RNA extracted was evaluated by RNA integrity number (RIN>7.3), calculated using an Agilent 2100 Bioanalyzer (Agilent Technologies) with the Agilent RNA 6000 Nano Kit. Overall design: For orthograft experiments, approximately 5 million murine prostate cancer cells (P1, CP1 and CP2) were injected in one of the anterior prostate lobes of male CD-1 Nude mice. Prostate orthograft tumours were used for RNA extraction and RNA seq.