CIL:12287, Homo sapiens, epithelial cell. In Cell Image Library
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MCF-10Aeco cells expressing GFP-α-tubulin and RFP-LL5α were seeded on glass-bottomed dishes at a low density and cultured overnight. Time-lapse images of the cells were collected at 2.5-min intervals for 8 h 6 min using a DeltaVision Core Systemdriven by SoftWoRx software (Applied Precision) equipped with an IX70 microscope with a Plan-Apochromat 100× NA 1.40 oil immersion objective (Olympus), a cooled charge-coupled device camera (CoolSNAP HQ2; Photometrics) and a CO2 incubator (Tokai Hit Co., Ltd.). GFP, Ex, 470; Em, 525; RFP, Ex, 572, Em, 632.
提供机构：
UC San Diego Library Digital Collections
创建时间：
2021-04-15
