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Lip-SMap analysis of traumatic acid in HT-22

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To explore potential targets of TA, LiP-SMap assay was used to identify the cellular proteins which could directly binding to TA. HT-22 cell lysates (three biological replicates for TA treatment) were incubated with TA or DMSO (vehicle), followed by limited proteolytic hydrolysis with proteases K and trypsin, and then identified by liquid chromatography-tandem mass spectrometry (LC-MS/MS).

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2023-12-01
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