Specifications and effects of heating devices.

Plant species and functional diversity play an important role in the stability and sustainability of grassland ecosystems. However, the changes and mechanisms of plant species and functional diversity under warming and nitrogen deposition are still unclear. In this study, we investigated the plant and soil characteristics of alpine meadows on the Qinghai-Tibet Plateau to explore the changes in species and functional diversity of plant communities under warming and nitrogen deposition, as well as their interrelationships and key determinants. The results showed that warming, nitrogen deposition, and their interactions had significant effects on plant species diversity (plant Shannon-Wiener index) and functional diversity (functional richness index, functional differentiation index, functional dispersion, and Rao’s quadratic entropy index). With the increase of warming and nitrogen deposition, the Shannon-Wiener index of plants increased first and then decreased. The plant functional richness index, functional diversity index, functional dispersion index, and Rao’s quadratic entropy index showed a decreasing trend. At the same time, with the increase in temperature and nitrogen deposition, the relationship between plant species diversity index and functional diversity index in the alpine meadow of Qinghai-Tibet Plateau gradually weakened. Redundancy analysis and structural equation modeling showed that both warming and nitrogen deposition had significant negative effects on the plant species diversity index and plant functional diversity index. Plant factors (Grasses importance value, leaf nitrogen weighted mean, specific leaf area-weighted mean, leaf area-weighted mean, and leaf weight weighted mean) and soil environmental factors (soil total nitrogen and soil carbon-nitrogen ratio) directly or indirectly affect plant community diversity under warming and nitrogen deposition.