five

Digestion and depletion of abundant proteins improves proteomic coverage.

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Data from Yates lab web site. Experiment: yeast control replicate 2, file: 120211_Yeast_Control2_Step02.mzml. Published as part of Nat Methods. 2013 Jan;10(1):54-6 9 . From the Abstract: {{i}} We exploited the abundance-dependent Michaelis-Menten kinetics of trypsin digestion to selectively digest and deplete abundant proteins with a method we call DigDeAPr. We validated the depletion mechanism with known yeast protein abundances, and we observed greater than threefold improvement in low-abundance human-protein identification and quantitation metrics. {{/i}}
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2014-07-02
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