RNA Sequencing of heart from near term fetuses (E18.5) of control and obese dams

The study objective was to determine differentially expressed mRNA transcripts in cardiac tissues from E18.5 fetal mice e born to obese dams fed a high fat/high sugar diet and control dams fed normal diet. Twelve-week-old C57BL/6J female mice were fed either a control diet (D12489, 10% calories from fat) or an obesogenic diet (Western Diet D12089B; Research Diets, 40% of calories from fat). Female mice fed the obesogenic diet had ad libitum access to 20% sucrose solution supplemented with micronutrients. Females were mated following a 25% gain in weight over the initial body weight in the obesogenic diet. All males were fed control diet. At E18.5, animals were euthanized and hearts were flash frozen. Following RNA isolation, RNAseq was performed.