COMPOSITIONS AND METHODS FOR PRODUCING POLYPEPTIDES WITH A MODIFIED GLYCOSYLATION PATTERN IN PLANT CELLS

A method of modifying a glycosylation pattern of a polypeptide-of-interest in a plant or plant cell is provided. The method comprising expressing in a plant or plant cell transformed to express at least one glycosidase in a subcellular compartment， a nucleic acid sequence encoding the polypeptide-of-interest， such that the at least one glycosidase and the polypeptide-of-interest are co-localized to the subcellular compartment of the plant or plant cell， thereby modifying the glycosylation pattern of the polypeptide-of-interest in the plant or plant cell. A method of modifying a glycosylation pattern of a polypeptide-of-interest in a plant or plant cell is provided. The method comprising expressing in a plant or plant cell transformed to express at least one glycosidase in a subcellular compartment， a nucleic acid sequence encoding the polypeptide-of-interest， such that the at least one glycosidase and the polypeptide-of-interest are co-localized to the subcellular compartment of the plant or plant cell， thereby modifying the glycosylation pattern of the polypeptide-of-interest in the plant or plant cell.