NanoImmunoERA. WP3 T3.1. Assay in body fluids

The present dataset contains data collected in the ecosystem of the NanoImmunoEra project, focusing on the development of an electrochemical DNA-based biosensor that enables rapid, reagentless measurement of plasma concentrations of the antibody drug trastuzumab. The approach supports real-time, in vivo monitoring in living animals (rats). Specifically, two distinct sensing architectures were systematically compared, a DNA scaffold sensor and an electrochemical aptamer-based sensor, before developing an optimized sensor in the latter class that, when challenged in undiluted whole blood, can measure over the entire clinical range of the drug. When implanted in animals, the sensor successfully tracks in vivo drug concentrations and clearly visualizes differences in drug exposure between individualrats following intravenous infusion.