TAF2 and MYC cooperate to promote hepatocellular carcinoma

In order to check the tumorigenic property of TAF2, either alone or in combination with MYC, we used Sleeping Beauty (SB) transposase-mediated somatic integration in combination with hydrodynamic tail vein injection (HTVI) technique in FVB mice. TAF2 alone did not induce tumor, MYC overexpression induced tumors at a variable levels, while both TAF2 and MYC overexpression resulted in profound development of HCC. Overall design: FVB/NJ mice (6 weeks old weighing approximately 20 g) from The Jackson Laboratory were used for hydrodynamic injection of plasmids. For each mouse pT3-EF1?H-TAF2 (20 microg) or pT3-EF1alphaH-MYC (20 microg) vector was mixed with pCMV-SB vector (0.8 microg), diluted in 2 ml of saline, sterilized with a 0.22 micron filter and injected via tail vein within 5-8 seconds. In combination of pT3-EF1alphaH-TAF2 (20 microg) and pT3-EF1alphaH-MYC (20 microg), 1.6 microg pCMV-SB was used. Five weeks after injection mice were sacrificed and tumors were subjected to RNA-seq