S1 File - Time matters: Transcriptomic insights into temporally regulated reproductive and physiological processes in the life cycle of salps

S1 Table. Overview of samples used for the de novo transcriptome of S. fusiformis. RNA concentration and quality of each sample was verified using NanoDropTM 2000 (Thermo Fisher Scientific, USA). RIN- a measure of integrity was measured by Bioanalyzer 2100 (Agilent Technologies, USA). S2 Table. Results of GO term enrichment analysis by topGO combined with results of DGE analysis by DESeq2: form comparison (campaign 1). S3 Table. Results of GO term enrichment analysis by topGO combined with results of DGE analysis by DESeq2: fertilized vs not fertilized (at TP1) (campaign 2). S4 Table. Results of GO term enrichment analysis by topGO combined with results of DGE analysis by DESeq2: fertilized vs not fertilized (at TP2) (campaign 2). S5 Table. Results of GO term enrichment analysis by topGO combined with results of DGE analysis by DESeq2: TP1 vs TP2 (fertilized) (campaign 2). S6 Table. Results of GO term enrichment analysis by topGO combined with results of DGE analysis by DESeq2: TP1 vs TP2 (unfertilized) (campaign 2). S7 Table. Results of GO term enrichment analysis by topGO combined with results of of DGE analysis by DESeq2: shared DEG of fertilized and unfertilized blastozooids (campaign 2). S8 Table. Results of GO term enrichment analysis by topGO combined with results of DGE analysis by DESeq2: main effect time (campaign 2). S9 Table. List of potential reference genes candidates used in ascidian studies and their mean expression, standard deviation and rank in the generated CV ranked list of S. fusiformis (approach 1). S10 Table. List of potential reference genes candidates detected in CV ranked list of S. fusiformis with <0.3 log fold change (approach 2). S11 Table. Raw Cq values of reference gene candidates after efficiency correction. S12 Table. Information of the candidate reference and target genes, including primer sequences, size, GC (%), melting temperature of primers and size of amplicons. Efficiency was calculated by E = 10(−1/slope). S13 Table. Expression stability values. S14 Table. Stability values and ranking of 6 reference gene candidates.
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