Expression of miRNAs in HOZOT, a line of multifunctional Treg cells

We used microarray analysis to compare miRNA expression profiles of novel type of Treg cell line, termed HOZOT, with those of activated T cells, so called conventional CD4+ T (Tconv) cells. HOZOT-17, a representative HOZOT cell line, was used for profiling. Tconv cells were prepared from the same UCB source as HOZOT-17. CD4+ cells were activated with anti-CD3 antibody plus anti-CD28 antibody (CD3/CD28) and cultured for at least seven days in the presence of IL-2. The chip used for this analysis was a mirVanaTM miRNA Bioarray system.