Functional assay of wild type and mutant inhibin and activin constructs as measured by FSHβ promoter activity in stably transfected LβT2 cells.

IC50: concentration of ligand causing 50% of the maximum inhibition. EC50: concentration of an agonist producing 50% of the maximum possible response. a,b,c,d represents differences between the groups, p<0.05 by F-test. A,B represents differences between the groups, p<0.05 by F-test. BG = betaglycan, H = human, Ch = chicken, wt = wild type. ext = N-terminal extension, PWR = Proline-rich wrist region, WHD = wrist helix deletion.