Multiomics Analyses Unveil Immune Landscape in Pediatric and Adult Sepsis [scRNA_seq]

Sepsis is a leading cause of global mortality, characterized by organ dysfunction due to a dysregulated immune response to infection. This study aims to delineate the immune landscape in sepsis through a combined analysis of single-cell RNA sequencing (scRNA-seq) with TCR and BCR sequencing. A total of 133 samples were analyzed, including PBMCs from healthy adults, adult ICU controls, adult sepsis patients, healthy children, pediatric ICU controls, and pediatric sepsis patients. The integration of scRNA-seq with TCR and BCR sequencing revealed both common and unique immune cell populations across different infection sources and between adults and children. These findings provide insights into the pathophysiology of sepsis and suggest potential targets for personalized therapeutic approaches. Peripheral blood mononuclear cells (PBMCs) were isolated from blood samples collected from healthy adult controls, adult ICU controls, adult sepsis patients, healthy pediatric controls, pediatric ICU controls, and pediatric sepsis patients. The sepsis patients, both adults and children, were categorized based on infections originating from different anatomical sites, including the abdomen, respiratory tract, urinary tract, skin, and central nervous system (CNS). Single-cell RNA sequencing, combined with TCR and BCR sequencing, was performed to analyze immune cell heterogeneity across these groups. *************************************************************** The raw sequencing have been uploaded to China Genomic Sequence Archive with accession number GSA:HRA008452. ***************************************************************