Transcriptome_analysis_of_mouse_pluripotency_genes. Transcriptome_analysis_of_mouse_pluripotency_genes
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下载链接：
https://www.ncbi.nlm.nih.gov/bioproject/PRJEB12223
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资源简介：
We aim to characterize transcriptional changes in mouse embryonic stem (ES) cells and mouse epiblast stem (EpiS) cells following the knockdown of essential genes identified in genome-wide RNAi screens. ES or EpiS cells were treated with esiRNA for 125 candidate genes and total RNA was collected from two biological replicates 72 hours post-transfection. The RNAs were normalized and arrayed in 96-well plates (500ng in 50 microlitres). Bar-coded libraries will be prepared from each 96-well plate and subjected to RNA-seq (Hiseq V4, 75 bp pair-end reads). This data is part of a pre-publication release. The data will be made available immediately via the ENA
创建时间：
2016-02-09
