Supplementary information for: Macrophage- and CD4+ T cell-derived SIV differ in glycosylation, infectivity, and neutralization sensitivity

The human immunodeficiency virus (HIV) envelope protein (Env) mediates viral entry into host cells and is the primary target for the humoral immune response. Env is extensively glycosylated, and these glycans shield underlying epitopes from neutralizing antibodies. The glycosylation of Env is influenced by the type of host cell in which the virus is produced. Thus, HIV is distinctly glycosylated by CD4+ T cells, the major target cells, and macrophages. However, the specific differences in glycosylation between viruses produced in these cell types have not been explored at the molecular level. Moreover, it remains unclear whether the production of HIV in CD4+ T cells or macrophages affects the efficiency of viral spread and resistance to neutralization. To address these questions, we employed the simian immunodeficiency virus (SIV) model. Glycan analysis implied higher relative levels of oligomannose-type N-glycans in SIV from CD4+ T cells (T-SIV) compared to SIV from macrophages (M-SIV)..., , , # Supplementary information for: Macrophage- and CD4+ T cell-derived SIV differ in glycosylation, infectivity, and neutralization sensitivity

[https://doi.org/10.5061/dryad.hmgqnk9rm](https://doi.org/10.5061/dryad.hmgqnk9rm)

## Description of the data and file structure

File names correspond to the figure in the manuscript for which the file supplies the underlying data. For instance, \"2BC_Data\" encompasses all data utilized in generating graphs for Fig. 2B and Fig. 2C. These data sets are stored as GraphPad Prism files and include details such as the number of biological replicates, raw data for each replicate, any data processing or statistical analysis performed, and the resulting graphs. Any exclusion of data points is accompanied by an explanation for this decision noted on the raw data sheets. Additionally, all data underlying the figures has been uploaded in Excel tables using file titles identical to the prism files.

Infectivity assays: Dataset 2A

Western blot with subseque...