five

Time-resolved cryo-EM on the CarH photoreceptor

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ESRF Portal2028-01-01 更新2026-04-23 收录
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https://doi.esrf.fr/10.15151/ESRF-ES-2298272407
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The purpose of this experiment is to collect six datasets on cryo-EM grids prepared in a time-resolved way: with a precisely controlled time delay between the initiation of a reaction (in this case the dissociation of a photosensitive protein, triggered with a light source) and vitrification. Our objective is to obtain high resolution maps of reaction intermediates in the light-induced dissociation of the photoreceptor CarH, and compare them with results obtained with crystallography techniques, in order to better understand its photo-reaction mechanism and eventually modify the protein for optogenetics purposes.
提供机构:
Institut de Biologie Structurale - IBS, 71 avenue des Martyrs, CS 10090, 38044 Grenoble Cedex 9, France
创建时间:
2028-01-01
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